Journal: Frontiers in Pharmacology

Article Title: Magnolol Attenuates Right Ventricular Hypertrophy and Fibrosis in Hypoxia-Induced Pulmonary Arterial Hypertensive Rats Through Inhibition of the JAK2/STAT3 Signaling Pathway

doi: 10.3389/fphar.2021.755077

Figure Lengend Snippet: Magnolol attenuated RV hypertrophy in hypoxia-induced PAH rats. (A) Representative images of HE staining for heart tissues. (B) Representative images of WGA staining for RV tissues. (C) The right ventricular wall thickness (calculated from HE staining). (D) The cross-sectional area of cardiomyocytes (calculated from WGA staining). (E) The perimeter of cardiomyocytes (calculated from WGA staining). (F) The mRNA levels of ANP in RV tissues. (G) The mRNA levels of BNP in RV tissues. ** p < 0.01 vs. Control; + p < 0.05, ++ p < 0.01 vs. Control; ## p < 0.01 vs. Vehicle.

Article Snippet: After washing 3 times with PBS, the slices were incubated with WGA staining solution (Servicebio, Wuhan, China) in the dark at 37°C for 30 min. DAPI staining solution was added to stain the nucleus for 5 min.

Techniques: Staining, Control

Journal: Cell Communication and Signaling : CCS

Article Title: TMEM100 acts as a TAK1 receptor that prevents pathological cardiac hypertrophy progression

doi: 10.1186/s12964-024-01816-2

Figure Lengend Snippet: TMEM100 overexpression attenuated cardiomyocytes enlargement and cardiac fibrosis in vivo . A Representative images of hematoxylin & eosin (HE) staining and wheat germ agglutinin (WGA) staining (left, n = 6) and LV cardiomyocytes’ cross-sectional-area (right, n = 120 cells) in every group. B The mRNA level of cardiac hypertrophic markers was analyzed by RT-PCR ( n = 4). C Representative images of picrosirius red (PSR) staining ( n = 6) and LV collagen volume (right) in every group. D The mRNA level of cardiac fibrosis markers was analyzed by RT-PCR ( n = 4). * p < 0.05 vs. AAV9-GFP sham, ** p < 0.01 vs. AAV9-GFP sham, # p < 0.05 vs. AAV9-GFP TAC, ## p < 0.01 vs. AAV9-GFP TAC

Article Snippet: For WGA staining, a diluted WGA dye solution was added, and the heart sectionslices were incubated at 37 °C in a light-free incubator for 1 h. Then, self-fluorescence quenching agent B was added (G1221, Servicebio), and the mixture was incubated for 5 min and washed with PBS.

Techniques: Over Expression, In Vivo, Staining, Reverse Transcription Polymerase Chain Reaction

Journal: Oncotarget

Article Title: Isolation of circulating tumor cells from pancreatic cancer by automated filtration

doi: 10.18632/oncotarget.21026

Figure Lengend Snippet: Red dots represent wild-type, blue dots the mutation, microwells with both signals are colored in green and microwells without signal yellow. (A) Detected cells were punched out from filter and relocated on the punch before WGA. (B) Amplified DNA was analyzed by digital PCR for KRAS G12D and KRAS G12V. Note higher background of leukocytes in DLA. Scale bars represent 200μm (filter) and 20μm (single cell).

Article Snippet: WGA-DNA solution was purified by Amicon Ultra 0.5mL centrifugal filter (Merck Millipore) .

Techniques: Mutagenesis, Amplification, Digital PCR